Fig. 3. Analyses of chimeric ASN genes.
(A) PCR analysis of chimeric genes. DNA samples were extracted from YKJ11 (WT) and each mutant cell (C1–C10), followed by PCR reactions using primers indicated as horizontal arrows in
Fig. 2A. (B) Growth of each mutant cell on SD plates lacking asparagine. YKJ11 (WT) and mutant cells containing chimeric genes (C1–C10) were spotted in 10-fold serial dilutions onto SD lacking asparagine and allowed to grow at 30°C for 3 days. (C) Sequence analysis of the chimeric genes. DNA sequence of
ASN1 is aligned with that of
ASN2 and base-pair differences are indicated to scale by black lines in the open box at the top of the graph. DNA sequences of the chimeric genes C1–C10 are represented by the lanes on the graph. Light and dark grey parts denote
ASN2 and
ASN1 sequences, respectively. The white areas indicate the junctions of gene fusion. Note that the junctions are the regions of fully matched sequences. N and C denote the deduced N- and C-terminal domains, respectively. Percentages denote levels of nucleotide identities.
© 2020 Korean J. Microbiol.
