Primers used for multiplex nested PCR
| Reaction | Target organism | Name | Sequence (5’ → 3’) | Annealing temp. (°C) | Amplicon size (bp) | References |
|---|---|---|---|---|---|---|
| 1st PCR | ACT-F1001 | CTG TCC TCC TAT TTT CAG TTG GTT | 54 | 630 | This study | |
| ACT-R1630 | GGC ATA TTT AGC AGG TTA AGG TC | |||||
| NF-F | GCC ACT GGA AAG TTT ACA AGG | 54 | 485 | This study | ||
| NF-R | GTT ATC TAC ACC CAA ATC ATG G | |||||
| 2nd PCR | Acant-F3 | AGG GTA ATG ATT AAT AGG GAT AGT TGG | 57 | 99 | Kim (2019) | |
| Acant-R4 | CAG ATG CTT TCG CAG AAG TTA AT | This study | ||||
| N.foul-F2 | GCC AGG CAG AGG TGT TTC | 57 | 210 | This study | ||
| N.foul-R1 | CCA CCG AAG GTG ATG AGA C |