Korean Journal of Microbiology

Indexed in /covered by CAS, KoreaScience & DOI/Crossref:eISSN 2383-9902   pISSN 0440-2413

Table. 1.

Table. 1.

Primers used for multiplex nested PCR

Reaction Target organism Name Sequence (5’ → 3’) Annealing temp. (°C) Amplicon size (bp) References
1st PCR Acanthamoeba spp. ACT-F1001 CTG TCC TCC TAT TTT CAG TTG GTT 54 630 This study
ACT-R1630 GGC ATA TTT AGC AGG TTA AGG TC
Naegleria spp. NF-F GCC ACT GGA AAG TTT ACA AGG 54 485 This study
NF-R GTT ATC TAC ACC CAA ATC ATG G
2nd PCR Acanthamoeba spp. Acant-F3 AGG GTA ATG ATT AAT AGG GAT AGT TGG 57 99 Kim (2019)
Acant-R4 CAG ATG CTT TCG CAG AAG TTA AT This study
N. fowleri N.foul-F2 GCC AGG CAG AGG TGT TTC 57 210 This study
N.foul-R1 CCA CCG AAG GTG ATG AGA C
Korean J. Microbiol. 2022;58:33-40 https://doi.org/10.7845/kjm.2022.1110
© 2022 Korean J. Microbiol.