Comparison of detection limit of multiplex PCR and multiplex nested PCR methods
Reaction | Target | Concentration (copies/reaction) | ||||||
---|---|---|---|---|---|---|---|---|
106 | 105 | 104 | 103 | 102 | 101 | 100 | ||
Multiplex PCR | + | + | + | + | + | - | - | |
+ | + | + | + | + | - | - | ||
Multiplex nested PCR | + | + | + | + | + | + | + | |
+ | + | + | + | + | + | + |