Korean Journal of Microbiology

Fig. 4.

Download original image
Fig. 4. The effect of Stephania brachyandra extract in LPS-induced gene transcriptional activation. (A) RAW264.7 cells were treated with S. brachyandra extract (20 or 100 µg/ml) in the presence of LPS (100 ng/ml) for 6 h. Phospho-IκB and β-actin protein levels were analyzed by Western blot analysis. (B) The relative levels of protein bands were measured by densitometry. (C, D) The NF-κB-luciferase (C) or the NFAT-luciferase (D) reporter vector was transfected into RAW264.7 cells. After 24 h, cells were incubated with the S. brachyandra extract (20 or 100 µg/ml) in the presence of LPS (100 ng/ml) for 6 h. Cell lysates were analyzed for luciferase activity. The results are presented as the means ±SD of three independent experiments. *p < 0.05, **p < 0.01.
Korean J. Microbiol. 2022;58:120-6 https://doi.org/10.7845/kjm.2022.2050
© 2022 Korean J. Microbiol.