Fig. 3. Effect of hydroxyurea on the MBF-dependent gene expression in S. pombe strains.
Quantitative real time PCR was performed to analyze the expression of MBF-dependent genes in Δlkh1 (lkh1 deletion strain), Δrad3 (rad3 deletion strain), and Δlkh1Δrad3 (lkh1 and rad3 double-deletion strain) cells with or without hydroxyurea (5 mM, 4 h) treatment. Primers specific to each gene were used. (*p < 0.05)
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