Fig. 4. Sensitivity of S. pombe strains to hydroxyurea and quantitative analysis of the rad3+ gene expression.
Serially diluted (10-fold) cells from mid-exponential phase of wild type, Δlkh1 (lkh1 deletion strain), Δtup11 (tup11 deletion strain), Δtup12 (tup12 deletion strain), Δtup11Δtup12 (tup11 and tup12 double-deletion strain) and Δprk1 (prk1 deletion strain) were plated onto the plates (A). Quantitative real time PCR was performed to confirm the expression of rad3+ in wild type, Δtup11, Δtup12 and Δprk1 cells with or without hydroxyurea treatment (5 mM, 4 h) (B). (***p < 0.001)
© 2022 Korean J. Microbiol.
