Fig. 5. Cellular accumulation of ROS in S. pombe strains.
Cells were stained with H2DCFDA to detect intracellular accumulation of ROS by hydroxyurea treatment. The wild type and Δlkh1 (lkh1 deletion strain) cell were grown to the early mid-exponential phase (OD600 = 0.3), followed by incubation at 30°C for 1 h with 50 μM H2DCFDA. Thereafter, hydroxyurea and hydrogen peroxide were treated and incubated at 30°C for 2 h. Accumulation of ROS in the cells was observed by fluorescence microscopy.
© 2022 Korean J. Microbiol.
