Fig. 4. (A) Time-course analysis of IsPETase expression. (B) Time-course analysis of NEXT-IsPETase expression. (C) Comparison of soluble NEXT-IsPETase expression under unoptimized and optimized conditions. Coomassie blue-stained SDS-PAGE gel (upper) and Western blot result using an anti-His6 antibody (lower) are shown. The arrows indicate the band position of each recombinant protein. Lanes: MW, molecular mass marker; S, soluble fraction; IS, insoluble fraction.

Korean J. Microbiol. 2023;59:16-23 https://doi.org/10.7845/kjm.2023.3010

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