Korean Journal of Microbiology

Fig. 4.

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Recombinant expression and purification of NEXT-RvCAs.
(A) Expression at 37°C. (B) Expression at 25°C. Coomassie blue-stained SDS-PAGE gels are shown. The band position of each recombinant protein is indicated with the red dash squares. (C) Purification of NEXT-RvCAs via His6-tag affinity chromatography. (D) Precipitation pattern of NEXT-RvCA5. The purified NEXT-RvCA5 was dialyzed against 20 mM phosphate buffer with or without 300 mM NaCl. Lanes: MW, molecular mass marker; T, Total cell lysate; S, soluble fraction; IS, insoluble fraction; FT, flow-through faction; P, purified protein; Sup, supernatant after centrifugation; Ppt, precipitate after centrifugation.
Korean J. Microbiol. 2023;59:204-13 https://doi.org/10.7845/kjm.2023.3071
© 2023 Korean J. Microbiol.