Korean Journal of Microbiology

Indexed in /covered by CAS, KoreaScience & DOI/Crossref:eISSN 2383-9902   pISSN 0440-2413

Table. 2.

Table. 2.

Physiological and biochemical characteristics and differential carbohydrate assimilation patterns of isolated Pseudomonas spp. using API 20NE (2.1) kit, API ZYM kit, and API 32GN kit

-, Negative; +, positive; w, weakly positive.

Isolated Pseudomonas strain

Pseudomonas mandelii GWJ2056 Pseudomonas canadensis GWJ2059 Pseudomonas thivervalensis GWJ2060 Pseudomonas baetica GWJ2123 Pseudomonas lurida GWJ2125 Pseudomonas brenneri JNP2044
API 20NE (2.1)
Nitrate reduction (NO3 → NO2-) + - - + - -
Reduction of nitrates to nitrogen - - + w - -
Arginine dihydrolase - + + - + +
Protease (gelatin hydrolysis) + - - - - +
D-Glucose + + + + + -
L-Arbinose - + + - + -
D-Mannose + + + + + -
N-Acetyl-D-glucosamine - + - w - -
Gluconate + + + + + -
Caprate - + + + + -
Adipate - - - - - -
Malate - + + w + -
Phenyl-acetate - - - w - -

API ZYM
Esterase (C4) + + + + + -
Esterase Lipase (C8) + + + + + -
Leucine arylamidase + - - + - +
Valine arylamidase - - + - - -
Acid phosphatase - - - - - +
Naphtol-AS-BI-phosphohydrolase + + - + + +
N–acetyl-β-glucosaminidase - + - + - -

API 32GN
D-Glucose + + + + + -
Salicin - + - - - -
D-Melibiose + + - - - -
L-Fucose - - - - - +
D-Sorbitol + + + + - -
L-Arabinose - + + - + -
Caprate - + + + + -
Valerate - - - - + -
2-Ketogluconate + - - + + +
4-Hydroxy-benzoate - + + + - -
L-Proline + - - - - +
L-Rhamnose - - - - - +
N-Acetyl-D-Glucosamine - + - + - -
D-Ribose + - - - - -
D-Maltose - + + - - -
Itaconate - - + - - -
Acetate + - + - + -
Lactate - - - - - +
L-Alanine + + + + - +
Glycogen - + - - - -
3-Hydroxy-benzoate + + - + - +
L-Serine + + + + + -

All isolated Pseudomonas strains resulted positive reaction on D-Mannitol, citrate and resulted negative reaction on indole production, glucose acidification, urease, β-glucosidase (esculin hydrolysis), β-galactosidase (PNPG), D-maltose, adipate of API 20NE kit (data not shown).

All isolated Pseudomonas strains resulted positive reaction on alkaline phosphatase and resulted negative reaction on lipase (C14), cystine arylamidase, trypsin, a-chymotrypsin, α-galactosidase, β-galactosidase, β-glucuronidase, α-glucosidase, β-glucosidase, a-mannosidase, a-fucosidase of API ZYM kit (data not shown).

All isolated Pseudomonas strains resulted positive reaction on D-mannitol, propionate, citrate, L-histidine, 3-hydroxy-butyrate and resulted negative reaction on inositol, D-sucrose, suberate, malonate, 5-ketogluconate of API 32GN kit (data not shown).

Korean J. Microbiol 2019;55:39-45 https://doi.org/10.7845/kjm.2019.8099
© 2019 Korean J. Microbiol