search for




 

Comparison between DNA- and cDNA-based gut microbial community analyses using 16S rRNA gene sequences§
Korean J. Microbiol 2019;55(3):220-225
Published online September 30, 2019
© 2019 The Microbiological Society of Korea.

Hyejun Jo, Jiwan Hong, and Tatsuya Unno*

Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, Jeju 63243, Republic of Korea
Correspondence to: *E-mail: tatsu@jejunu.ac.kr; Tel.: +82-64-754-3354; Fax: +82-64-756-3351
These authors contributed equally to the study.
Received June 14, 2019; Revised July 3, 2019; Accepted July 5, 2019.
Abstract
Studies based on microbial community analyses have increased in the recent decade since the development of next generation sequencing technology. Associations of gut microbiota with host's health are one of the major outcomes of microbial ecology filed. The major approach for microbial community analysis includes the sequencing of variable regions of 16S rRNA genes, which does not provide the information of bacterial activities. Here, we conducted RNA-based microbial community analysis and compared results obtained from DNA- and its cDNA-based microbial community analyses. Our results indicated that these two approaches differed in the ratio of Firmicutes and Bacteroidetes, known as an obesity indicator, as well as abundance of some key bacteria in gut metabolisms such as butyrate producers and probiotics strains. Therefore, cDNA-based microbial community may provide different insights regarding roles of gut microbiota compared to the previous studies where DNA-based microbial community analyses were performed.
Keywords : cDNA, gut microbiota, microbial community analysis, miSeq


September 2019, 55 (3)
Full Text(PDF) Free

Social Network Service
Services

Author ORCID Information