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Isolation and characterization of cellulolytic yeast belonging to Moesziomyces sp. from the gut of Grasshopper§
Korean J. Microbiol 2019;55(3):234-241
Published online September 30, 2019
© 2019 The Microbiological Society of Korea.

Ju-Young Kim1, Hee-Young Jung2,3, Jong-Seok Park4, Sung-Jin Cho4, Hoon Bok Lee1, Gi-Ho Sung5,6*, Gayathri Subramani1*, and Myung Kyum Kim1*

1Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 01797, Republic of Korea
2College of Agricultural and Life Sciences, Kyungpook National University, Daegu 41566, Republic of Korea
3Institute of Plant Medicine, Kyungpook National University, Daegu 41566, Republic of Korea
4School of Biological Sciences, College of Natural Sciences, Chungbuk National University, Chungbuk, 28644, Republic of Korea
5Institute for Healthcare and Life Science, International St. Mary's Hospital and College of Medicine, Catholic Kwandong University, Incheon 22711, Republic of Korea
6Department of Microbiology, College of Medicine, Catholic Kwandong University, Gangneung 25601, Republic of Korea
Correspondence to: *(G.H. Sung) E-mail: sung97330@gmail.com; Tel.: +82-032-290-2772; Fax: +82-2-290-2614 /
(G. Subramani) E-mail: drgaya@swu.ac.kr; Tel.: +82-2-970-5670; Fax: +82-2-970-5974 /
(M.K. Kim) E-mail: biotech@swu.ac.kr; Tel.: +82-2-970-5667; Fax: +82-2-970-5974
Received June 10, 2019; Revised September 4, 2019; Accepted September 4, 2019.
Abstract
An intensive interaction between yeasts and insects has highlighted their relevance for attraction to food and for the insect's development and behavior. Yeast associated in the gut of insects secretes cellulase which aided in the food digestion (cellulose degradation). Three strains of cellulose-degrading yeast were isolated from the gut of adult grasshoppers collected in Gyeonggi Province, South Korea. The strains ON22T, G10T, and G15T, showed positive cellulolytic activity in the carboxymethyl cellulose (CMC)-plate assay. The phylogenetic tree based on sequence analysis of D1/D2 domains of the large subunit rRNA gene and the internal transcribed spacer (ITS) regions revealed that the strains ON22T (100 and 98.4% sequence similarities in D1/D2 domains and ITS) and G10T (99.8 and 99.5% in D1/D2 domain and ITS region) were most closely related to the species Moesziomyces aphidis JCM 10318T; G15T (100% in D1/D2 domains and ITS) belongs to the species Moesziomyces antarcticus JCM 10317T, respectively. Morphology and biochemical test results are provided in the species description. Cellulase with its massive applicability has been used in various industrial processes such as biofuels like bioethanol productions. Therefore, this is the first report of the cellulolytic yeast strains ON22T, G10T, and G15T related to the genus Moesziomyces in the family Ustilaginaceae (Ustilaginales), in Korea.
Keywords : Moesziomyces sp., D1/D2 domain, grasshopper, ITS, rRNA, yeast


September 2019, 55 (3)
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