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Characterization of Miniimonas sp. S16 isolated from activated sludge
Korean J. Microbiol 2019;55(3):242-247
Published online September 30, 2019
© 2019 The Microbiological Society of Korea.

Hyeon-Woo Koh1, Hongik Kim1*, and Soo-Je Park2*

1R&D Division, VITABIO, Inc., Daejeon 34025, Republic of Korea
2Department of Biology, Jeju National University, Jeju 63243, Republic of Korea
Correspondence to: *(H. Kim) E-mail:; Tel.: +82-42-864-4647; Fax: +82-42-864-4645 /
(S.J. Park) E-mail:; Tel.: +82-64-753-3524; Fax: +82-64-756-3541
Received July 5, 2019; Revised July 31, 2019; Accepted August 1, 2019.
Biological factors (e.g. microorganism activity) in wastewater treatment plant (WWTP) play essential roles for degradation and/or removal of organic matters. In this study, to understand the microbial functional roles in WWTP, we tried to isolate and characterize a bacterial strain from activated sludge sample. Strain S16 was isolated from the activated sludge of a municipal WWTP in Daejeon metropolitan city, the Republic of Korea. The cells were a Gram-stain-positive, non-motile, facultative anaerobe, and rod-shaped. Strain S16 grew at a temperature of 15~40°C (optimum, 30°C), with 0~9.0% (w/v) NaCl (optimum, 1.0~2.0%), and at pH 5.5~9.0 (optimum, pH 7.0~7.5). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S16 was most closely related to the unique species Miniimonas arenae NBRC 106267T (99.79%, 16S rRNA gene sequence similarity) of the genus Miniimonas. The cell wall contained alanine, glutamic acid, serine, and ornithine. Although the isolation source of the type strain NBRC 106267T which considered as a marine microorganism is sea sand, that of strain S16 is terrestrial environment. It might raise an ecological question for habitat transition. Therefore, comparative genome analysis will be valuable investigation for shedding light on their potential metabolic traits and genomic streamlining.
Keywords : Miniimonas, polyphasic analysis, sludge

September 2019, 55 (3)