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Comparison of gut microbial diversity of breast-fed and formula-fed infants
Korean J. Microbiol 2019;55(3):268-273
Published online September 30, 2019
© 2019 The Microbiological Society of Korea.

Kyeong Soon Kim1, Jung Shin1, JiSoo Sim1, SuJi Yeon2, Pyeong An Lee2, and Moon Gyu Chung1*

1Korea Research Institute of Bio-medical Science, Daejeon 34946, Republic of Korea
2Korea ND Lab Co., Ltd., Jeonju 54863, Republic of Korea
Correspondence to: *E-mail: pgaeguri@hanmail.net; Tel.: +82-42-716-2310; Fax: +82-42-716-2309
Received July 17, 2019; Revised August 23, 2019; Accepted August 26, 2019.
Abstract
The intestinal microbiomes vary according to the factors such environment, age and diet. The purpose of this study was to compare the gut microbial diversity between Korean infants receiving breast-fed milk and formula-fed milk. We analyzed microbial communities in stool samples collected from 80 Korean infants using next generation sequencing. Phylum level analysis revealed that microbial communities in both breast-fed infants group (BIG) was dominated by Actinobacteria (74.22 ± 3.48%). Interestingly, the phylum Actinobacteria was dominant in formula-fed infants group A (FIG-A) at 73.46 ± 4.12%, but the proportions of phylum Actinobacteria were lower in formulafed infants group B and C (FIG-B and FIG-C) at 66.52 ± 5.80% and 68.88 ± 4.33%. The most abundant genus in the BIG, FIG-A, FIG-B, and FIG-C was Bifidobacterium, comprising 73.09 ± 2.31%, 72.25 ± 4.93%, 63.81 ± 6.05%, and 67.42 ± 5.36% of the total bacteria. Furthermore, the dominant bifidobacterial species detected in BIG and FIG-A was Bifidobacterium longum at 68.77 ± 6.07% and 66.85 ± 4.99% of the total bacteria. In contrast, the proportions of B. longum of FIG-B and FIG-C were 58.94 ± 6.20% and 61.86 ± 5.31% of the total bacteria. FIG-A showed a community similar to BIG, which may be due to the inclusion of galactooligosaccharide, galactosyllactose, synergy-oligosaccharide, bifidooligo and improvement material of gut microbiota contained in formula-milk. We conclude that 5-Bifidus factor contained in milk powder promotes the growth of Bifidobacterium genus in the intestines.
Keywords : Bifidobacterium longum, 5-Bifidus factor, breast-fed, formula-fed, next generation sequencing