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Complete genome sequence of Celluosilyticum lentocellum WCF-2 isolated from cow dung
Korean J. Microbiol 2019;55(3):313-315
Published online September 30, 2019
© 2019 The Microbiological Society of Korea.

Jun Heo, Jaehong You, InCheol Park, Byeong-Hak Han, Soon-Wo Kwon, and Jae-Hyung Ahn*

Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of Korea
Correspondence to: *E-mail: hyungz@korea.kr; Tel.: +82-63-238-3045; Fax: +82-63-238-3834
Received September 9, 2019; Revised September 18, 2019; Accepted September 18, 2019.
Abstract
An anaerobic bacterial strain WCF-2 was isolated from cow dung in finding cellulose-degrading bacteria for use as silage additives. Strain WCF-2 showed a higher cellulolytic activity than Cellulosilyticum lentocellum DSM 5427T, the closest relative of strain WCF-2 (98.2% of 16S rRNA gene sequence similarity). We sequenced the complete genome of strain WCF-2 and compared it with that of C. lentocellum DSM 5427T. The OrthoANI value between the two strains was 97.9% thus strain WCF-2 was identified as C. lentocellum. The genome size of strain WCF-2 was 4,779,774 bp with a G + C content of 34.4%, 4,154 coding genes (CDS), 54 pseudo genes, and 142 RNA genes. Strain WCF-2 harbored seven cellulase genes, five of which showed low similarities with those of C. lentocellum DSM 5427T.
Keywords : Cellulosilyticum lentocellum, cellulolytic activity, genome sequence


September 2019, 55 (3)
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