The genus Rossellomorea, belonging to the family Bacillaceae, has been recent proposed by Gupta and distinguished from all other Bacillaceae species by the presence of exclusively shared conserved signature indels (Gupta et al., 2020). Type species of Rosellomorea, Rossellomorea aquimaris, was isolated from tidal flat (Yoon et al., 2003). At the time of writing, the genus comprises up to five validly named species, including R. aquimaris. Rossellomorea marisflavi from sea water of a tidal flat of the Yellow Sea in Korea (Gupta et al., 2020), R. arthrocnemi from roots of plant (Navarro-Torre et al., 2021), and R. vietnamensis from Vietnamese fish sauce (Noguchi et al., 2004). Rossellomorea oryzaecorticis was previously isolated as a moderately halophilic bacterium species from the rice husks (Hong et al., 2014). Here, we report the complete genome sequence of Rossellomorea oryzaecorticis KS-H15a.

Strain KS-H15a (KACC 22746) was isolated from tidal flat sediments in Shinan-gun (34.747742 N 125.932485 E), South Korea. Cultures of strain KS-H15a was obtained on Marine Agar (MA, BD) at 37°C. Genomic DNA was extracted from plate culture, grown on 37°C for 3 days in MA, using Wizard Genomic DNA Purification Kit (Promega) according to the manufacturer’s instructions. The genome of Rossellomorea oryzaecorticis KS-H15a was sequenced by NovaSeq^TM 6000 (Illumina) and the PacBio sequel (Pacific Bioscience) system at Macrogen for short and long reads, respectively. The total of 939,371,378 bp with 108,678 reads (N₅₀, 10,493 bp) were produced by the PacBio sequel platform and de novo assembly was performed by Canu v1.7 (Koren et al., 2017). An assembled contig was corrected using filtered short reads from illumina (total of 702,803,790 bp with 4,656,762 reads) and then annotations were performed by Pilon v1.21 (Walker et al., 2014) and Prokka v1.1.0 (Seemann, 2014) software, respectively. Aragon (v1.2.36) was used to predict tRNAs, and Barrnap software (v0.6) was used to predict rRNAs (5S rRNAs, 16S rRNAs, and 23S rRNAs) (Lagesen et al., 2007). The resulting circular genome has a length of 4,409,168 bp (190-fold coverage), with a GC content of 44.0%. Clustering of functional genes derived from the genome of R. oryzaecorticis KS-H15a was performed using the USEARCH program against the COG database within BPGA (Edgar, 2010). and the portions of genes assigned to each COG category were expressed as relative percentages. Default parameters were used for all software unless otherwise specified.

Genome features of Rossellomorea oryzaecorticis strain KS-H15a are presented in Table 1. Genome annotation was performed using NCBI Prokaryotic Genome Annotation Pipeline software. The complete genome sequence of R. oryzaecorticis KS-H15a has 148 RNAs (110 tRNAs, 33 rRNAs, and 5 non-coding RNAs), 4,347 protein coding genes, and 31 pseudogenes. Approximately 70% of the protein coding genes of strain KS-H15a were assigned to COGs. Functional genes belonging to COG categories involved in housekeeping processes including general function prediction only (R, 13%), amino acid transport and metabolism (E, 9.2%), and transcription (K, 8.8%) were enriched in the genome of R. oryzaecorticis KS-H15a. The genome contained oxidative stress response resistance related genes such as inorganic pyrophosphatase (M1J35_00550), thioredoxin (M1J35_18625), thiol peroxidase (M1J35_20375), glutaredoxin (M1J35_22265). A cluster of heat shock proteins: HtpX (M1J35_08105), molecular chaperone DnaJ (M1J35_17730), DnaK (M1J35_17735), GrpE (M1J35_17740), chaperonin GroEL (M1J35_04205) and GroES (M1J35_04200) was detected in the genome. In addition, L-lactate dehydrogenase (M1J35_09460) was found which means it is candidate lactic acid bacteria.

Table 1

Genome features	Value
Genome size (bp)	4,409,168
G + C content (%)	44.0
Total genes	4,526
Protein coding genes	4,347
No. of tRNAs	110
No. of rRNAs (5S, 16S, 23S)	11, 11, 11
No. of ncRNAs	5
Pseudo-genes	31
GenBank Accession No.	CP096886

Nucleotide sequence accession number

The complete genome sequence and raw sequencing reads for strain KS-H15a were deposited under GenBank accession numbers CP096886. The associated BioProject and BioSample accession numbers are PRJNA832433 and SAMN27863079, respectively.

Rossellomorea oryzaecorticis는 그람 양성, 호기성, 내생포자를 형성하는 중호염성 막대균이다. 대한민국 신안군의 갯벌로부터 R. oryzaecorticis KS-H15a (= KACC 22746) 균주를 분리하였다. Rossellomorea oryzaecorticis KS-H15의 전장 유전체 염기서열은 4,409,168 bp 크기로 G + C 함량은 44.0%였다. 전장 유전체는 4,347개의 단백질을 암호화하는 유전자와 110개의 전달 RNA (tRNA) 및 리보솜 RNA (rRNA)를 보유하고 있다.

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT; [2021R1C1C1013796]).

The authors have no conflict of interest to report.