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Nisaea acidiphila sp. nov., isolated from a marine algal debris, emended description of the genus Nisaea Urios et al. 2008, and the emendation of Thalassobaculum salexigens as Thalassobaculum litoreum subsp. salexigens comb. nov.
Korean J. Microbiol. 2023;59(1):58-59
Published online March 31, 2023
© 2023 The Microbiological Society of Korea.

Kae Kyoung Kwon1,2*, Ji-Hye Oh1#, Sung-Hyun Yang1, Mi-Jeong Park1, and Yeonju Lee1,2

1Marine Biotechnology Research Center, Korea Institute of Ocean Science and Technology, Busan 49111, Republic of Korea
2Major of Marine Technology and Convergence Engineering, KIOST School, KIOST School, University of Science and Technology, Daejeon 34113, Republic of Korea
#Present address: Gyeongin Regional Office of Food and Drug Safety, Gwacheon 13809, Republic of Korea

한국미생물학회의 국문학술지인 미생물학회지(58권 4호, pp. 255–265)에 출간된 상기 논문의 ‘Description of Nisaea acidiphila sp. nov.’ 와 국문 적요의 균주 기탁 정보를 ‘MEBiC11861T (= KCCM 43219T = JCM 31589T)’와 같이 정정함을 알려 드립니다.

In the article by Kwon et al. published in Korean Journal of Microbiology 2022; 58, 255-265, The culture collection numbers of type strain in the ‘Description of Nisaea acidiphila sp. nov.’ and in the ‘Korean Abstract’ should be corrected as MEBiC11861T (= KCCM 43219T = JCM 31589T).

The sentence in ‘Description of Nisaea acidiphila sp. nov.’ and in ‘Korean Abstract’ should be read as follows;

Description of Nisaea acidiphila sp. nov.

Nisaea acidiphila (' N.L. n. acidum acid from L. adj. acidus sour; Gr. adj. philos loving; N.L. fem. adj. acidiphila acid-loving)

In addition to the characteristics of the genus, the species characteristic based on the type strain MEBiC11861T is as follows. The Cells are rod-shaped with 1.4 ± 0.5 μm × 0.5 ± 0.2 μm in width. Colonies are cream-coloured with circular, convex, opaque and butyrous with entire edges, and 0.5–1.0 μm in diameter on marine agar after 2–3 days cultivation. Growth observed at temperature between 10 and 42°C (optimum 26–29°C), pH between 4.0 and 8.5 (optimum pH 5.0) and presence of 0–10% (w/v, optimum 0.5%) NaCl. No growth was observed at 4°C and 46°C, with 11% NaCl, and at pH 3.5 and 9.0. Nitrate is reduced to nitrite. Produces acetoin but could not produce H2S and indole. Oxidase, catalase, β-galactosidase, and β-glucosidase activities are present but gelatinase and urease activities are negative in API20E and 20NE kits. When assayed with the API ZYM system alkaline- and acidphosphatases, leucine-, valine-, and cystine arylamidases, esterase (C4), esterase lipase (C8), lipase (C14), β-galactosidase, α- and β-glucosidases, α-mannosidase, and naphthol-AS-BIphosphohydrolase activities are present. Assimilates glucose, arabinose, mannose, mannitol, and maltose in API 20E kit. Oxidizes Tween 80, L-fucose, α-D-glucose, D-galactose, m-inositol, D-raffinose, D-galacturonic acid, D-gluconic acid, g-hydroxy butyric acid, α-keto glutaric acid, D,L-lactic acid, propionic acid, glucuron amide, L-arabinose, gentiobiose, α-D-lactose, lactulose, mannose, β-methyl-D-glucoside, D-psicose, L-rhamnose, D-sorbitol, sucrose, D-trehalose, turanose, xylitol, methyl pyruvate, cis-aconitic acid, citrate, D-galactonic acid lactone, D-glucosaminic acid, D-glucuronic acid, β-hydroxy butyric acid, itaconic acid, malonic acid, quinic acid, Dsaccharic acid, succinic acid, succinamic acid, alaninamide, L-alanine, L-asparagine, L-aspartic acid, L-glutamic acid, glycyl-L-aspartic acid, hydroxy-L-proline, L-leucine, L-ornithine, L-phenylalanine, L-proline, L-pyroglutamic acid, L-serine, and D,L-carnitine on Microlog GN2 plate. Major fatty acids are C12:0, C16:0, C12:0 3-OH, summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), summed feature 8 (C18:1 ω7c and/or C18:1 ω6c), and C19:0 cyclo ω8c when grown at 25°C. The predominant polar lipids are phosphatidyl glycerol, phosphatidylethanolamine, two unidentified lipids, two unidentified aminophospholipids, and one unidentified aminolipid. The DNA G + C content is 63.0 mol%.

Type strain MEBiC11861T (= KCCM 43219T = JCM 31589T) was isolated from an algal debris collected at Kosrae State, Federation State of Micronesia. The GenBank/DDBJ/EMBL accession number for the 16S rRNA gene is KX364080 and that of the genome is CP102480.

적 요

그람 음성의 통성 혐기성의 이동성을 지닌 간균(1.4 ± 0.46 μm × 0.53 ± 0.17 μm) MEBiC11861T은 미크로네시아 연방 코스래주(162°57'23.1''E, 5°21'13.0''N)에서 채취된 미동정 해조류 잔해에서 분리되었다. 16S rRNA 유전자 서열 분석 결과 MEBiC11861T 균주는 Nisaea속 균주들과 높은 유사성(97.0~98.4%)을 보였다. MEBiC11861T 균주는 섭씨 10~42°C (최적 26~29°C), pH 4.0~8.5 (최적 pH 5.0) 및 0~10% (최적 0.5%) NaCl 농도 조건에서 성장이 관찰되었다. 주요 세포 지방산은 C12:0 (5.6%), C16:0 (29.0%), C12:0 3-OH (4.3%), summed feature 3(C16:1 ω7c and/or C16:1 ω6c; 9.9%), summed feature 8 (C18:1 ω7c and/or C18:1 ω6c; 31.2%) 그리고 C19:0 cyclo ω8c (10.6%) 였으며 DNA G + C 함량은 65.6 mol%이다. 주요 호흡기 퀴논은 Q-10이며 글루콘산염, 말린산염, 아디핀산염, 아라비노스 등을 탄소원으로 이용한다. DNA G + C 비율, 세포지방산 조성, pH 및 염도의 성장 범위 등 여러 표현형 특성이 Nisaea속의 다른 균주들과 구별된다. 이와 같은 다상분류 결과에 기초하여 균주 MEBiC11861TNisaea속의 새로운 종으로 분류되어야 함을 제안하며 이를 Nisaea acidipila sp. nov.로 제안하였다. 표준균주는 MEBiC11861T (= KCCM 43219T = JCM 31589T)이다. 균주 MEBiC11861T 균주에서 확인된 새로운 사실들을 감안하여 Nisaea Urios et al. 2008에 대한 개정 설명과 Thalassobaculum salexigensT. litoreum subsp. salexigens로의 재분류제안도 제시하였다.

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March 2023, 59 (1)
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