한국미생물학회의 국문학술지인 미생물학회지(58권 4호, pp. 255–265)에 출간된 상기 논문의 ‘Description of Nisaea acidiphila sp. nov.’ 와 국문 적요의 균주 기탁 정보를 ‘MEBiC11861^T (= KCCM 43219^T = JCM 31589^T)’와 같이 정정함을 알려 드립니다.

In the article by Kwon et al. published in Korean Journal of Microbiology 2022; 58, 255-265, The culture collection numbers of type strain in the ‘Description of Nisaea acidiphila sp. nov.’ and in the ‘Korean Abstract’ should be corrected as MEBiC11861^T (= KCCM 43219^T = JCM 31589^T).

The sentence in ‘Description of Nisaea acidiphila sp. nov.’ and in ‘Korean Abstract’ should be read as follows;

Description of Nisaea acidiphila sp. nov.

Nisaea acidiphila (a.ci.di'phi.la. N.L. n. acidum acid from L. adj. acidus sour; Gr. adj. philos loving; N.L. fem. adj. acidiphila acid-loving)

In addition to the characteristics of the genus, the species characteristic based on the type strain MEBiC11861^T is as follows. The Cells are rod-shaped with 1.4 ± 0.5 μm × 0.5 ± 0.2 μm in width. Colonies are cream-coloured with circular, convex, opaque and butyrous with entire edges, and 0.5–1.0 μm in diameter on marine agar after 2–3 days cultivation. Growth observed at temperature between 10 and 42°C (optimum 26–29°C), pH between 4.0 and 8.5 (optimum pH 5.0) and presence of 0–10% (w/v, optimum 0.5%) NaCl. No growth was observed at 4°C and 46°C, with 11% NaCl, and at pH 3.5 and 9.0. Nitrate is reduced to nitrite. Produces acetoin but could not produce H2S and indole. Oxidase, catalase, β-galactosidase, and β-glucosidase activities are present but gelatinase and urease activities are negative in API20E and 20NE kits. When assayed with the API ZYM system alkaline- and acidphosphatases, leucine-, valine-, and cystine arylamidases, esterase (C4), esterase lipase (C8), lipase (C14), β-galactosidase, α- and β-glucosidases, α-mannosidase, and naphthol-AS-BIphosphohydrolase activities are present. Assimilates glucose, arabinose, mannose, mannitol, and maltose in API 20E kit. Oxidizes Tween 80, L-fucose, α-D-glucose, D-galactose, m-inositol, D-raffinose, D-galacturonic acid, D-gluconic acid, g-hydroxy butyric acid, α-keto glutaric acid, D,L-lactic acid, propionic acid, glucuron amide, L-arabinose, gentiobiose, α-D-lactose, lactulose, mannose, β-methyl-D-glucoside, D-psicose, L-rhamnose, D-sorbitol, sucrose, D-trehalose, turanose, xylitol, methyl pyruvate, cis-aconitic acid, citrate, D-galactonic acid lactone, D-glucosaminic acid, D-glucuronic acid, β-hydroxy butyric acid, itaconic acid, malonic acid, quinic acid, Dsaccharic acid, succinic acid, succinamic acid, alaninamide, L-alanine, L-asparagine, L-aspartic acid, L-glutamic acid, glycyl-L-aspartic acid, hydroxy-L-proline, L-leucine, L-ornithine, L-phenylalanine, L-proline, L-pyroglutamic acid, L-serine, and D,L-carnitine on Microlog GN2 plate. Major fatty acids are C_12:0, C_16:0, C_12:0 3-OH, summed feature 3 (C_16:1 ω7c and/or C_16:1 ω6c), summed feature 8 (C_18:1 ω7c and/or C_18:1 ω6c), and C_19:0 cyclo ω8c when grown at 25°C. The predominant polar lipids are phosphatidyl glycerol, phosphatidylethanolamine, two unidentified lipids, two unidentified aminophospholipids, and one unidentified aminolipid. The DNA G + C content is 63.0 mol%.

Type strain MEBiC11861^T (= KCCM 43219^T = JCM 31589^T) was isolated from an algal debris collected at Kosrae State, Federation State of Micronesia. The GenBank/DDBJ/EMBL accession number for the 16S rRNA gene is KX364080 and that of the genome is CP102480.

그람 음성의 통성 혐기성의 이동성을 지닌 간균(1.4 ± 0.46 μm × 0.53 ± 0.17 μm) MEBiC11861^T은 미크로네시아 연방 코스래주(162°57'23.1''E, 5°21'13.0''N)에서 채취된 미동정 해조류 잔해에서 분리되었다. 16S rRNA 유전자 서열 분석 결과 MEBiC11861^T 균주는 Nisaea속 균주들과 높은 유사성(97.0~98.4%)을 보였다. MEBiC11861^T 균주는 섭씨 10~42°C (최적 26~29°C), pH 4.0~8.5 (최적 pH 5.0) 및 0~10% (최적 0.5%) NaCl 농도 조건에서 성장이 관찰되었다. 주요 세포 지방산은 C_12:0 (5.6%), C_16:0 (29.0%), C_12:0 3-OH (4.3%), summed feature 3(C_16:1 ω7c and/or C_16:1 ω6c; 9.9%), summed feature 8 (C_18:1 ω7c and/or C_18:1 ω6c; 31.2%) 그리고 C_19:0 cyclo ω8c (10.6%) 였으며 DNA G + C 함량은 65.6 mol%이다. 주요 호흡기 퀴논은 Q-10이며 글루콘산염, 말린산염, 아디핀산염, 아라비노스 등을 탄소원으로 이용한다. DNA G + C 비율, 세포지방산 조성, pH 및 염도의 성장 범위 등 여러 표현형 특성이 Nisaea속의 다른 균주들과 구별된다. 이와 같은 다상분류 결과에 기초하여 균주 MEBiC11861^T는 Nisaea속의 새로운 종으로 분류되어야 함을 제안하며 이를 Nisaea acidipila sp. nov.로 제안하였다. 표준균주는 MEBiC11861^T (= KCCM 43219^T = JCM 31589^T)이다. 균주 MEBiC11861^T 균주에서 확인된 새로운 사실들을 감안하여 Nisaea Urios et al. 2008에 대한 개정 설명과 Thalassobaculum salexigens를 T. litoreum subsp. salexigens로의 재분류제안도 제시하였다.

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