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Fission yeast Pci2 has function in mRNA export as a component of TREX-2
Korean J. Microbiol 2018;54(4):325-329
Published online December 31, 2018
© 2018 The Microbiological Society of Korea.

Jin Hee Park, and Jin Ho Yoon*

Department of Biotechnology, Sungshin Women’s University, Seoul 01133, Republic of Korea
Correspondence to: E-mail: jhoyoon@sungshin.ac.kr; Tel.: +82-2-920-7675; Fax: +82-2-920-2047
Received November 26, 2018; Revised December 4, 2018; Accepted December 5, 2018.
Abstract

Thp1/PCID2, PCI domain-containing protein, is a component of the evolutionally conserved TREX-2 complex linking mRNA transcription and export. In fission yeast, Schizosaccharomyces pombe, the pci2 (SPBC1105.07c) gene encodes a PCI domain- containing protein that is predicted as a fission yeast orthologue of Thp1 (in budding yeast)/PCID2 (in human). Repression of pci2 expression inhibited both growth and mRNA export. And over-expression of pci2 also exhibited growth retardation with slight accumulation of poly(A)+ RNA in the nucleus. Moreover, yeast two-hybrid and co-immunoprecipitation analysis showed that the Pci2 protein physically interacted with Sac3 and Dss1, which are members of TREX-2 complex. These observations support that the S. pombe Pci2 protein, as a component of TREX-2 complex, is implicated in mRNA export.

Keywords : Schizosaccharomyces pombe, mRNA export, Pci2, TREX-2 complex
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December 2018, 54 (4)