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Antimicrobial activities of Bacillus subtilis DS660 and Paenibacillus polymyxa DS842
Korean J. Microbiol 2018;54(4):354-361
Published online December 31, 2018
© 2018 The Microbiological Society of Korea.

Da-Sol Lee, and Hong-Gyu Song*

Department of Biological Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea
Correspondence to: E-mail: hgsong@kangwon.ac.kr; Tel.: +82-33-250-8545; Fax: +82-33-259-5665
Received August 6, 2018; Revised October 5, 2018; Accepted October 19, 2018.
Abstract

This study was carried out to isolate soil bacteria with antimicrobial activity and evaluate antimicrobial substances produced by isolated bacteria. Among many isolates Bacillus subtilis DS660 and Paenibacillus polymyxa DS842 showed high antimicrobial activities against 6 species of microbial residents on human skin and 3 species of pathogenic bacteria. DS660 and DS842 showed 15.3~26.8 and 11.3~27.5 mm of inhibition zone diameter, respectively on nutrient agar medium against most target bacteria and fungi. DS660 and DS842 produced 57 ± 8 and 170 ± 15 μmol/ml of siderophore, respectively as an antimicrobial substance. Analysis of ethyl acetate extract of culture supernatants of DS660 and DS842 suggested production of glycolipid biosurfactant which reduced surface tension of culture supernatant of DS660 and DS842 from 60.0 to 40.3 and 30.3 mN/m, respectively. DS660 and DS842 also showed 169.2 ± 9.9 and 357.2 ± 13.7 nmol/min/mg protein of β-1,3-glucanase activity, respectively, and hydrolyzed cell wall components of 3 bacterial species. These results suggest that B. subtilis DS660 and P. polymyxa DS842 may be utilized as an environment-friendly biocontrol agent against some skin microbes and pathogenic bacteria.

Keywords : Bacillus subtilis DS660, β-1,3-glucanase, Paenibacillus polymyxa DS842, antimicrobial activity, biosurfactant, siderophore
Body
재료 및 방법
결과 및 고찰
적 요
감사의 말
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December 2018, 54 (4)