
A Gram-positive, rod-shaped, ivory colored, and motile,
During a positive activity screening for β-glucosidase, a Gram-positive, rod-shaped, and motile bacterium Lactobacillus koreensis 26-25 was isolated from Korean traditional food kimchi. The ginsenoside conversion ability test of the L. koreensis 26-25 was determined by the described method of Siddiqi et al. (2017). Therefore, complete genome sequencing was applied to strain 26-25 for further genome base study. This strain is available from the host institute and Korean Agricultural Culture Collection (= KACC 92257P).
The genomic DNA of L. koreensis 26-25 was extracted and purified with the Genomic-tip system 100/G (QIAGEN). The genome was sequenced by using the Pacific Biosciences RSII Sequencing method manual. The general aspects of library construction and sequencing can be found at the JGI website (https://www.jgi.doe.gov). Sequence reads were assembled using PacBio SMRT Analysis (version 2.3.0) with default options. The genome sequence was annotated by using the NCBI Prokaryotic Genome Automatic Annotation Pipeline (PGAP, http://www.ncbi.nlm.nih.gov/books/NBK174280/). The rRNAs and tRNAs were predicted by using rRNAmmer and tRNAscan-SE, respectively.
The complete genome of L. koreensis 26-25 consists of a single circular chromosome of 3,006,812 bp, with 49.23% G + C content. Of the 2,720 predicted genes, 2,556 protein-coding genes, 18 rRNA genes (5S, 16S, and 23S), 60 tRNA genes, and 85 pseudogenes were also identified (Fig. 1). The majority of the protein-coding genes (96.04%) were assigned a putative function, while the remaining predicted genes were annotated as hypothetical or conserved hypothetical proteins. The genome statistics are described in Table 1.
Analysis of the complete genome of L. koreensis 26-25 showed that it encodes glycosides and hydrolases, including 3 β-glucosidases, 5 α-glucosidases, 7 α-L-arabinofuranosidases, and 2 β-xylosidases that may be responsible for its ability to convert ginseng saponins (Siddiqi et al., 2017). In addition, the genome annotation also revealed other useful genes, phosphohydrolase, alcohol dehydrogenase (ADH), permease related with exporter of polyketide antibiotics and sucrose phosphotransferase.
The availability of the complete genome sequence of L. koreensis 26-25 will allow further functional and comparative genome analyses to better understand the genomic traits involved in the conversion of plant secondary metabolites, as described by Siddiqi et al. (2017).
The complete genome sequence of Lactobacillus koreensis 26-25 has been deposited in DDBJ/EMBL/NCBI GenBank under accession number CP012033.
김치로부터 분리한 Lactobacillus koreensis 26-25 균주의 유전체서열을 분석하였다. 균주 26-25의 유전체는 G + C 비율이 49.23%이며, 2,720개의 유전자와 2,556개의 단백질 코딩 유전자, 85개의 위유전자 그리고 78개의 RNA 유전자를 포함한 단일 원형 염색체로 구성되었으면 그 크기는 3,006,812 bp였다. 균주 26-25는 인삼사포닌의 당 분해에 관여하는 여러 타입의 글라이코시다제 유전자를 가지고 있었다. 이러한 지놈 분석은 주요 진세노사이드가 우수한 약리학적 활성의 미량 진세노사이드로 전환하는데 관여하는 유전자 특징을 이해하는데 큰 기여가 되었다.
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2018R1D1A1B07045774).
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