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Removal of mycotoxin ochratoxin A by isolated bacteria
Korean J. Microbiol 2019;55(1):33-38
Published online March 31, 2019
© 2019 The Microbiological Society of Korea.

Ho-Yeong Choi and Hong-Gyu Song*

Department of Biological Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea
Correspondence to: E-mail: hgsong@kangwon.ac.kr; Tel.: +82-33-250-8545; Fax: +82-33-259-5665
Received November 19, 2018; Revised January 2, 2019; Accepted January 3, 2019.
Abstract
Ochratoxin A (OTA), one of mycotoxins produced mainly by Aspergillus is a common contaminant of stored grains, posing health hazards to human and livestock. The aim of this study is to explore ability of isolated bacteria Bacillus subtilis AF13 and Streptomyces shenzhenensis YR226 to remove OTA. AF13 and YR226 could remove 94.23 and 97.73% of OTA (100 μg/L), respectively during 24 h incubation in NB medium. When cultures of two strains were separated into washed cells and cell-free supernatant, the supernatant of both strains removed more than 90% of 100 μg/L OTA, and 98.88% of OTA could be also removed by the washed cells of YR226. OTA removal occurred in a few second by the supernatant of both strains, and treatments of autoclaving, proteinase K and chymotrypsin did not affect the OTA removal by the culture supernatants, which indicate that some thermostable and non-proteinaceous substances secreted by these bacteria may be involved in OTA removal in these two bacteria. These results suggest that AF13 and YR226 can be used to remove OTA from OTA-contaminated grains and feeds, and therefore decrease economic damage in agriculture and feed industry.
Keywords : Bacillus subtilis AF13, Streptomyces shenzhenensis YR226, mycotoxin, ochratoxin A (OTA), OTA removal


March 2019, 55 (1)