Schematic diagram of pUC119 expression vector for expression of MassM2 in Escherichia coli.
The MassM2 ScFv antibody was cloned into pUC119 expression vector containing a 5′-pelB leader sequence, leucine zipper motif and 3′-hexahistidine (His6) tag at C-terminus of ScFv. The murine IgG3 hinge domain was inserted for the connection of ScFv to leucine zipper domain.
© 2022 Korean J. Microbiol.
