Two-dimensional thin-layer chromatography of the total polar lipids of strain 2012CJ34-3T.
Chloroform-methanol-water (65:25:4, by vol.) was used in the first direction, followed by chloroform-acetic acid-methanol-water (40:7.5:6:2, by vol.) in the second direction. The following spray reagents were used for detection: (a) 5% ethanoic molybdophosphoric acid (for total lipids); (b) molybdenum blue (Sigma) (for phosphorus-containing polar lipids); (c) 2% ninhydrin (for amino lipids); (d) α-naphthol (Sigma) (for glycolipis). PE, phosphatidylethanolamine; APL, unidentified aminophospholipid; AL, unidentified aminolipid; GL, unidentified glycolipid; L, unidentified lipids.
© 2022 Korean J. Microbiol.
