Effect of chaperone co-expression on (A) IsPETase and (B) NEXT-IsPETase expression at 25℃ (left) and 37℃ (right).
Coomassie blue-stained SDS-PAGE gel (upper) and Western blot result using an anti-His6 antibody (lower) are shown. The arrows indicate the band position of each recombinant protein. Lanes: MW, molecular mass marker; S, soluble fraction; IS, insoluble fraction; P, purified protein.
© 2023 Korean J. Microbiol.
