Korean Journal of Microbiology

Fig. 2.

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The transmembrane domain of LapB is indispensable for its function.
(A) Complementation of growth defects of the lapB mutant in M9 minimal media containing energy-poor carbon sources. The wild-type (closed circles and triangles), lapB mutant (open circles and triangles), and lapB mutant harboring pRE1-LapB (slashed circles and triangles) cells grown in LB medium overnight were inoculated in M9 minimal medium containing α-ketoglutarate (circles) or fumarate (triangles) as a carbon source. Cell growth was recorded by measuring the optical density at 600 nm. (B) Requirement of the transmembrane domain of LapB in complementation of the phenotypes of the lapB mutant. The indicated strain cells were serially diluted from 108 cells/ml to 104 cells/ml in 10-fold serial dilutions and spotted onto LB plates under indicated stress conditions or M9 minimal medium plates containing 0.2% α-ketoglutarate and fumarate as a carbon source. Besides plates presenting incubation temperature, all plates were incubated at 37°C.
Korean J. Microbiol. 2023;59:173-83 https://doi.org/10.7845/kjm.2023.3069
© 2023 Korean J. Microbiol.