Korean Journal of Microbiology

Fig. 3.

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Suppression of the phenotypes of the lapB mutant by depletion of PBP1b or LpoB.
(A) Suppression of the phenotypes of the lapB mutant by depletion of PBP1b. The indicated strain cells were serially diluted from 108 cells/ml to 104 cells/ml in 10-fold serial dilutions and spotted onto LB plates under indicated stress conditions or M9 minimal medium plates containing indicated molecules as a carbon source. All plates were incubated at 37°C. (B) LpxC levels in the lapB mutant and lapB mrcB double mutant cells. The wild-type (1), lapB mutant (2), lapB mrcB double mutant (3), and MG1655/pBAD-LpxC (4) cells were grown in an LB medium containing 0.2% arabinose. Western blot analysis with anti-LpxC and anti-FtsZ antibodies was carried out using 2 × 107 cells in MG1655/pBAD-LpxC and 2 × 108 cells in other strains, harvested at the early exponential phase. The arrow indicates LpxC proteins. (C) Suppression of the phenotypes of the lapB mutant by depletion of LpoB. The indicated strain cells were serially diluted from 108 cells/ml to 104 cells/ml in 10-fold serial dilutions and spotted onto LB plates without or with indicated chemicals, or an alkaline LB plate. Besides a plate presenting incubation temperature, all plates were incubated at 37°C.
Korean J. Microbiol. 2023;59:173-83 https://doi.org/10.7845/kjm.2023.3069
© 2023 Korean J. Microbiol.