Korean Journal of Microbiology

Table. 4.

Kinetic parameters for in vitro aminoacylation of Sc TyrRS and AzPheRS mutants

TyrRS AzPheRS-1 AzPheRS-2 AzPheRS-3




+ Tyr + AzPhe + Tyr + AzPhe + Tyr + AzPhe + Tyr + AzPhe
Km (μM) 18 n.d. 119 112 122 108 124 116
kcat (min-1) 114 n.d. 57 103 71 126 76 142
kcat/Km 6.31 n.d. 0.48 0.92 0.58 1.17 0.61 1.23
Korean J. Microbiol. 2024;60:1-11 https://doi.org/10.7845/kjm.2024.4009
© 2024 Korean J. Microbiol.