In vivo amber suppression activity of AzPheRS mutants is dependent on AzPhe.
(A) Chloramphenicol-resistant growth depends on AzPhe. Bacteria transformed with pACamG plus an aaRS expression plasmid as indicated were cultured in GMML media. After 48 h culture in the presence or absence of 1 mM AzPhe, the chloramphenicol-resistant growth of bacteria was measured. (B) The activities of β-galactosidase produced by amber suppression in DH10B (Tn:lacZam) were compared among bacteria containing either Sc TyrRS or AzPheRS mutants in the presence or absence of AzPhe. *P < 0.01; ** P < 0.05.
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