Summary of oligonucleotide primers used in this study
| Primers | Nucleotide sequence (5’ → 3’) | Usage |
|---|---|---|
| SDM-1 | CATTTGAAATTA |
Site-directed mutagenesis, Y43T |
| SDM-2 | GTTGGCTCTTCT |
Site-directed mutagenesis, Y127F |
| SDM-3 | GCAAGCGTTG |
Site-directed mutagenesis, D177P, I178L, and L181G |
| SDM-4 | GCAAGCGTTG |
Site-directed mutagenesis, D177P, I178L, and L181Q |
| SDM-5 | GATAGCGTCAGCAACAATTTTTAG |
Site-directed mutagenesis, P320A and D321A |
| E-3 | CAATCGAGCTCATGTCCTCTGCTGCCACGGTTGAC | Forward primer for generation of pACamG-AzPheRS-1, -2, or -3 |
| E-5 | CAAGCCTGCAGTTACAATTTGGTTTCCTCTAGTTTCG | Reverse primer for generation of pACamG-AzPheRS-1, -2, or -3 |