Korean Journal of Microbiology

Fig. 2.

Download original image
Experimental scheme. For the induction of adipocyte differentiation, 3T3-L1 preadipocytes were seeded. At confluence (day 0), the cultured preadipocytes were induced to differentiate by the addition of differentiating medium containing 115 μg/ml methylisobutylxanthine (IBMX), 10 μg/ml insulin, and 1 μM dexamethasone (Dex) from day 0 to day 2. At day 2, medium was changed with medium containing 10 μg/ml insulin for an additional 4 days from day 2 to day 6. The medium was refreshed every 2 days. At day 6, differentiated 3T3-L1 cells were incubated with MC or BMC or 1 µM insulin for 15 minutes and then assayed for glucose uptake activities (a). Mature adipocytes were used for Real-time PCR analysis. MC or BMC were added to the cell culture medium at concentration of 2% from day 0 to day 2 (b). DMEM, Dulbecco’s modified Eagle’s medium. DMEM/F12, Dulbecco’s modified Eagle medium/Nutrient Mixture F-12.
Korean J. Microbiol. 2024;60:12-25 https://doi.org/10.7845/kjm.2024.4002
© 2024 Korean J. Microbiol.