qPCR conditions for 16S rRNA,
Primer | Initial denaturation | Cycling condition | Final extension | Number cycle | ||
---|---|---|---|---|---|---|
Denaturation | Annealing | Extension | ||||
16S rRNA | 95°C for 2 min | 95°C for 30 sec | 53°C for 2 min | 72°C for 2 min | 72°C for 2 min | 35 |
37°C for 15 min | 95°C for 10 min | 95°C for 2 min | 57°C for 35 sec | 72°C for 40 sec | 42 | |
37°C for 15 min | 95°C for 10 min | 95°C for 30 sec | 58°C for 30 sec | 72°C for 30 sec | 45 |