Korean Journal of Microbiology

Table. 2.

qPCR conditions for 16S rRNA, blaTEM and blaOXA genes for all bacteria isolate subjected in this study

Primer Initial denaturation Cycling condition Final extension Number cycle

Denaturation Annealing Extension
16S rRNA 95°C for 2 min 95°C for 30 sec 53°C for 2 min 72°C for 2 min 72°C for 2 min 35
blaTEM 37°C for 15 min 95°C for 10 min 95°C for 2 min 57°C for 35 sec 72°C for 40 sec 42
blaOXA 37°C for 15 min 95°C for 10 min 95°C for 30 sec 58°C for 30 sec 72°C for 30 sec 45
Korean J. Microbiol. 2024;60:152-60 https://doi.org/10.7845/kjm.2024.4059
© 2024 Korean J. Microbiol.