The Effect on growth and mRNA export of deletion and over-expression of S. pombe mep33.
(A) mep33 knockout cells have a slight growth retardation. The entire mep33 coding region was replaced for the marker genes, ura4+. Wild-type (WT) and mep33 deletion cells (△mep33) were serially diluted 10-fold, spotted onto YES plates, and incubated for 3 days at 30°C. (B) Poly(A)+ RNA localization of wild-type (WT) and mep33 deletion (△mep33) mutant. Cells were grown to log phase at 30°C and prepared for FISH. The nucleus is indicated by DAPI staining in the right panels. (C) Over-expression of mep33 shows no growth defect. Cells overexpressing wild-type mep33 under the control of strong nmt1 promoter were spotted on EMM plate without thiamine. pREP3X vector alone served as the negative control.
© 2024 Korean J. Microbiol.
