Korean Journal of Microbiology

Primers for Legionella species identification and virulence gene typing

Target gene	Primer name	Sequence (5' to 3')	Size (bp)	Reference
16S rRNA	16S-F	AGGGTTGATAGGTTAAGAGC	386	Mirkalantari et al. (2020)
16S rRNA	16S-R	CCAACAGCTAGTTGACATCG	386

mip	mip1	GGTGACTGCGGCTGTTATGG	632
mip	mip2	GGCCAATAGGTCCGCCAACG	632

dotA	dot1	CAAATCCGGCATTCAAAATC	174
dotA	dot2	CTATTGTCGCCTTGGGTGTT	174

hsp60	hsp1	GCGAATCGTTGTTACCAAAGAAAAC	401
hsp60	hsp2	CAATTTGACGCATTGGAGATTCAATAG	401

prpA	lvh1	GTTTTAATCCCCCAGCAAGC	260	Samrakandi et al. (2002)
prpA	lvh2	AATATCCCTACTCATCCTCG	260

lvhB3,4	lvh3	GGCTAGGAGGTTCTTGTG	1kb
lvhB3,4	lvh4	ATTGGCCGAGATGTCCTT	1kb

lvhB8,9	lvh5	CCTCTACGCATTACAACGCC	280
lvhB8,9	lvh6	GTGGTGGTAAAGGGAATGCC	280

rtxA1	rtx1	GATCCGCAAGTAGCGCTCAC	630
rtxA1	rtx2	TGTAATGCTGGCATTAGGCG	630

rtxA2	rtx3	CTGATGCTGCTACGGAACAC	540
rtxA2	rtx4	CCGCAGTCATTACACCTGCG	540

enhC	enh1	AATGCTTTGTATGCCCTCGG	438
enhC	enh2	CATATCAGCGCTTTGGCCATC	438